Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody produced in mouse, clone DI-22, purified immunoglobulin, buffered aqueous solution

Application

Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules). It has also been used for enzyme immunoassay (EIA).

Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin.

Immunogen

Digoxin-KLH.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl₂ and 15 mM sodium azide

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.